2011年9月15日

Mir193b-365 is essential for brown fat differentiation

Mir193b-365 is essential for brown fat differentiation

Sun L, Xie H, Mori MA, Alexander R, Yuan B, Hattangadi SM, Liu Q, Kahn CR, Lodish HF

Nat Cell Biol. 2011 Jul 10

• Mir193b-365 is a bicistronic transcript miRNA enriched in brown fat tissue.

• The function of Mir193b-365 is general regulator of adipogenesis in white and brown fat cells and supporting the development of brown fat cells by regulating several adipogenesis markers ( Adipoq, Cebpα, Fabp4 and PPARγ) and brown fat-enriched markers (PPARα, PGC-1α, Dio2, Ucp1, PRDM16 and Cidea).

• Mir193b directly interacts with Runx1t1 mRNA as predicted target site and downregulation of Runx1t1 partially explains the role of Mir193b during adipogenesis.

• Mir193b-365 can directly repress myogenesis, thereby contributing to the regulation of brown fat versus muscle lineage determination.

• Mir193b directly interacts with Cdon and Igfbp5 as predicted target sites which are previously implicated as pro-myogenic factors.

• Mir193b can induce brown fat adipogenesis in C2C12 myoblast cells by upregulating adipogenic factor (PPARγ and Cebpα) and promoting common adipogenesis marker and brown fat selective marker, and also repressing myogenic marker (Pax3 and MyoD)

• Mir193b-365 is regulated by PRDM16 by inducing expression of PPARα/RXR (retinoid-X receptor) binding sites within segment of Mir193b.

担当：Royhan